ACE-2 Antibody (AC18F) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80035

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AC18F

Format

Azide and BSA Free

Concentration

1 mg/ml

View all ACE-2 Antibodies »

Product Specifications

Immunogen

Recombinant human ACE-2.

Specificity

Recognizes human ACE-2. Does not detect recombinant human ACE-2 that has a tag at the C-terminus of the protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ACE-2 Antibody (AC18F) - Azide and BSA Free

Western Blot: ACE-2 Antibody (AC18F)Azide and BSA Free [NBP2-80035]

Western Blot: ACE-2 Antibody (AC18F)Azide and BSA Free [NBP2-80035]

Western Blot: ACE-2 Antibody (AC18F) [NBP2-80035] - Western blot analysis using anti-ACE2 (human), mAb (AC18F) at 1: 2,000 dilution. Lane 1. hACE2 (Fc protein). Lane 2. hACE2 (Ecto domain) (FLAG(R)-tagged). Lane 3. Other hGITR (Fc protein) (control).
Immunohistochemistry-Paraffin: ACE-2 Antibody (AC18F) - Azide and BSA Free [NBP2-80035]

Immunohistochemistry-Paraffin: ACE-2 Antibody (AC18F) - Azide and BSA Free [NBP2-80035]

Immunohistochemistry-Paraffin: ACE-2 Antibody (AC18F) [NBP2-80035] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Mouse Anti-Human ACE-2 (AC18F) Monoclonal Antibody (Catalog # NBP2-80035) at 10 ug/ml overnight at 4 degrees C. Detection kit: VC001. Counterstain: Hematoxylin. Localization: respiratory bronchioles.
Flow Cytometry: ACE-2 Antibody (AC18F) - Azide and BSA Free [NBP2-80035]

Flow Cytometry: ACE-2 Antibody (AC18F) - Azide and BSA Free [NBP2-80035]

Flow Cytometry: ACE-2 Antibody (AC18F) [NBP2-80035] - Detection of endogenous human ACE2 by anti-ACE2 (h), mAb (AC18F). HepG2 cell line treated with CellTase solution (Abeomics) before analysis and then stained with anti-ACE2 (h), mAb (AC18F) (red line) or an isotype control, mouse IgG1 (green line) at 1ug/10E6 cells each, revealed with a PE-conjugated goat anti-mouse IgG and then analyzed by flow cytometry.
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Applications for ACE-2 Antibody (AC18F) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:2000 - 1:10000

Flow Cytometry

1:1000

Western Blot

1:2000 - 1:10000
Application Notes
Western blot: Use ECL substrate. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.

Reviewed Applications

Read 1 review rated 1 using NBP2-80035 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um-filtered solution in PBS, pH 7.4, 10% glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACE-2

Angiotensin I Converting Enzyme 2 (ACE2), also known as ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family (theoretical molecular weight 92 kDa). The predicted mouse ACE2 protein sequence consists of 798 amino acids, including an N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. Mouse ACE2 is 40% homologous in amino acid identity to the N- and C-terminal domains of mouse somatic ACE. Enzymatically, ACE2 converts the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts Ang I to Ang II.

Genetic data from Drosophila, mice and rats show that the ACE2 protein is an essential regulator of heart function in vivo. ACE2 mRNA is found at high levels in testis, kidney and heart with moderate levels in colon, small intestine, and ovary. The ACE2 protein contributes to organ function through the renin-angiotensin system, playing a central role in vascular, renal, and myocardial physiology.

Virology research has demonstrated that the severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein binds to its functional receptor, ACE2 (1). Vimentin is a type III intermediate filament protein expressed in mesenchymal cells that helps comprise the cytoskeleton in all animal cells. Studies have demonstrated that vimentin allows cell binding that allows the uptake of SARS-CoV spike protein into a host (2). This direct interaction of SARS-CoV with vimentin has been identified as an entry mechanism for the virus into a host, mediated by the SARS-CoV receptor ACE2 (1,2). It has been shown that ACE2 is the SARS-CoV-2 receptor required for cell entry and plays a physiological role in the replication of SARS-CoV in an infected host (1). Studies using human ACE2 antibody demonstrated a blockade of SARS-CoV-2 and ACE2 interaction, indicating an important physiological component of viral transmission and potential anti-viral therapeutic strategies (3).

References

1. Li, W., Moore, M. J., Vasilieva, N., Sui, J., Wong, S. K., Berne, M. A., . . . Farzan, M. (2003). Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature, 426(6965), 450-454. doi:10.1038/nature02145

2. Yu YT, Chien SC, Chen IY, Lai CT, Tsay YG, Chang SC, Chang MF. (2016) Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci. doi: 10.1186/s12929-016-0234-7

3. Hoffmann, M., Kleine-Weber, H., Schroeder, S., Kruger, N., Herrler, T., Erichsen, S., . . . Pohlmann, S. (2020). SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. doi:10.1016/j.cell.2020.02.052

Long Name

Angiotensin I Converting Enzyme 2

Alternate Names

ACE2, ACEH

Gene Symbol

ACE2

Additional ACE-2 Products

Product Documents for ACE-2 Antibody (AC18F) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ACE-2 Antibody (AC18F) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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