Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Goat, Rabbit, Zebrafish

Cited:

Mouse, Bovine

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 22D3

Format

BSA Free

Concentration

1 mg/ml

View all alpha-Cardiac Actin Antibodies »

Product Specifications

Immunogen

Derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of Actin Alpha 1 Cardiac Muscle with an acetylated N-terminus (Ac-DDEETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.

Reactivity Notes

The epitope recognized by this antibody is highly conserved; the antibody therefore cross-reacts with many species

Bovine reactivity reported in scientific literature (PMID: 30591979). Mouse reactivity reported in scientific literature (PMID: 28624189).

Specificity

This antibody is highly specific for actin alpha 1 (cardiac), and does not cross react with other actin isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Scientific Data Images for Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free

Immunocytochemistry/ Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free [NBP1-97725]

Immunocytochemistry/ Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free [NBP1-97725]

Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing somites in stage 46 Xenopus laevis embryo using Actin Alpha 1 Cardiac Muscle Antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free [NBP1-97725]

Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free [NBP1-97725]

Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Paraffin section of human cardiac muscle immunostained with 22D3 (1:500)
Immunocytochemistry/ Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free [NBP1-97725]

Immunocytochemistry/ Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free [NBP1-97725]

Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo
View 1 more images

Applications for Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:5000
Application Notes
This antibody is suitable for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titration; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting applications..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-97725 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, pH 7.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Cardiac Actin

Actin, alpha cardiac muscle 1 (ACTC1) is a 377 aa cytoskeletal filament in cardiac muscle which interacts with the 50-KDa domain of the myosin motor domain in cardiac muscle contraction. Actin is highly conserved across species, and differs from skeletal muscle actin (ACTA1) by only four amino acids. Mutations in the ACTC1 gene are linked to familial hypertrophy cardiomyopathy, atrial septal defects, arrhythmia, and chronic inflammatory cardiomyopathy.

Long Name

Actin, Alpha, Cardiac Muscle 1

Alternate Names

ACTC, ACTC1, ASD5, Cardiac alpha-Actin, CMD1R, CMH11, LVNC4

Gene Symbol

ACTC1

Additional alpha-Cardiac Actin Products

Product Documents for Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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