Actin Alpha 1 Cardiac Muscle Antibody (22D3) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97725CL1
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine, Goat, Rabbit, Zebrafish
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # 22D3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of Actin Alpha 1 Cardiac Muscle with an acetylated N-terminus (Ac-DDEETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Reactivity Notes
The epitope recognized by this antibody is highly conserved; the antibody therefore cross-reacts with many species
Bovine reactivity reported in scientific literature (PMID: 30591979). Mouse reactivity reported in scientific literature (PMID: 28624189).
Bovine reactivity reported in scientific literature (PMID: 30591979). Mouse reactivity reported in scientific literature (PMID: 28624189).
Specificity
This antibody is highly specific for actin alpha 1 (cardiac), and does not cross react with other actin isoforms.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Actin Alpha 1 Cardiac Muscle Antibody (22D3) [CoraFluor™ 1]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined..
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: alpha-Cardiac Actin
The Actin protein, a component of the eukaryotic cytoskeleton, is highly conserved between multiple species. Actin is a globular protein of approximately 42kDa. Levels of the Actin protein may vary between different cell types, but are consistent within a given cell type. For this reason, Actin protein levels are often considered a 'house-keeper', an internal control for protein loading.
The actin network represents an essential component of the cell cytoskeleton, which is made up of microfilaments composed of linear assemblies of actin molecules. Actin assembly is known to be important for the remodeling of cell shape during cell motility, cell division and morphogenesis. The biological functions of actin occur in concert with a large number of Actin binding proteins, including cofilin, actin depolymerization factor (ADF), myosins, and many more.
Actin molecules accumulate as Hirano bodies, in neurons of patients with Alzheimer's disease, Creutzfeldt-Jakob disease, and other neurodegenerative diseases. Hirano bodies are intracellular aggregates of actin and actin-associated proteins.
Long Name
Actin, Alpha, Cardiac Muscle 1
Alternate Names
ACTC, ACTC1, ASD5, Cardiac alpha-Actin, CMD1R, CMH11, LVNC4
Gene Symbol
ACTC1
Additional alpha-Cardiac Actin Products
Product Documents for Actin Alpha 1 Cardiac Muscle Antibody (22D3) [CoraFluor™ 1]
Product Specific Notices for Actin Alpha 1 Cardiac Muscle Antibody (22D3) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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