Actin Alpha 1 Cardiac Muscle Antibody (22D3) [DyLight 350]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97725UV
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine, Goat, Rabbit, Zebrafish
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
DyLight 350 (Excitation = 353 nm, Emission = 432 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 22D3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of Actin Alpha 1 Cardiac Muscle with an acetylated N-terminus (Ac-DDEETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Reactivity Notes
The epitope recognized by this antibody is highly conserved; the antibody therefore cross-reacts with many species
Bovine reactivity reported in scientific literature (PMID: 30591979). Mouse reactivity reported in scientific literature (PMID: 28624189).
Bovine reactivity reported in scientific literature (PMID: 30591979). Mouse reactivity reported in scientific literature (PMID: 28624189).
Specificity
This antibody is highly specific for actin alpha 1 (cardiac), and does not cross react with other actin isoforms.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Applications for Actin Alpha 1 Cardiac Muscle Antibody (22D3) [DyLight 350]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: alpha-Cardiac Actin
Long Name
Actin, Alpha, Cardiac Muscle 1
Alternate Names
ACTC, ACTC1, ASD5, Cardiac alpha-Actin, CMD1R, CMH11, LVNC4
Gene Symbol
ACTC1
Additional alpha-Cardiac Actin Products
Product Documents for Actin Alpha 1 Cardiac Muscle Antibody (22D3) [DyLight 350]
Product Specific Notices for Actin Alpha 1 Cardiac Muscle Antibody (22D3) [DyLight 350]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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