Key Product Details

Species Reactivity

Human, Rat, Porcine, Goat, Rabbit

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3B3

Format

BSA Free

Concentration

1 mg/ml

View all Actin Antibodies »

Product Specifications

Immunogen

This antibody is a mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.

Reactivity Notes

The epitope recognized by Clone 3B3 is highly conserved and the antibody is therefore expected to react with many other species.

Specificity

This antibody highly specific for alpha-skeletal actin, and does not cross react with other actin isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Scientific Data Images for Actin Antibody (3B3) - BSA Free

Immunohistochemistry-Paraffin: Actin Antibody (3B3) - BSA Free [NBP1-97723]

Immunohistochemistry-Paraffin: Actin Antibody (3B3) - BSA Free [NBP1-97723]

Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Striated muscle from human tongue immunostained with 3B3 (1250).
Immunohistochemistry-Paraffin: Actin Antibody (3B3) - BSA Free [NBP1-97723]

Immunohistochemistry-Paraffin: Actin Antibody (3B3) - BSA Free [NBP1-97723]

Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Paraffin sections of human striated skeletal muscle.

Applications for Actin Antibody (3B3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:5000
Application Notes
This antibody is suitable for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titration; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting applications..

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, pH 7.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Actin

Actin is a 43kDa filament protien that is ubiquitously expressed by all eukaryotic cells. This wide expression makes Actin an ideal "housekeeping gene", which may even account for up to 50% of total cellular protein. Actin microfilaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. alpha Actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. Actin antibodies are most commonly used as controls for protein standardization or in cytoskeletan or cell cycle research.

Alternate Names

ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, Alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3

Gene Symbol

ACTA1

Additional Actin Products

Product Documents for Actin Antibody (3B3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Actin Antibody (3B3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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