Actin Antibody (ACTA1/360)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13759

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # ACTA1/360

Concentration

0.2 mg/ml

View all Actin Antibodies »

Product Summary for Actin Antibody (ACTA1/360)

Immunogen

Recombinant human Actin protein (Uniprot: P68133)

Reactivity Notes

Predicted to show a broad reactivity.

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14162)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Actin Antibody (ACTA1/360)

Immunohistochemistry-Paraffin: Actin Antibody (ACTA1/360) [NBP3-13759]

Immunohistochemistry-Paraffin: Actin Antibody (ACTA1/360) [NBP3-13759]

Immunohistochemistry-Paraffin: Actin Antibody (ACTA1/360) [NBP3-13759] - Formalin-fixed, paraffin-embedded human skeletal muscle stained with Actin antibody (ACTA1/360). Inset: PBS instead of primary antibody; secondary only negative control.
Immunohistochemistry-Paraffin: Actin Antibody (ACTA1/360) [NBP3-13759]

Immunohistochemistry-Paraffin: Actin Antibody (ACTA1/360) [NBP3-13759]

Immunohistochemistry-Paraffin: Actin Antibody (ACTA1/360) [NBP3-13759] - Formalin-fixed, paraffin-embedded human skeletal muscle stained with Actin antibody (ACTA1/360). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. AB, 5min.

Applications for Actin Antibody (ACTA1/360)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Actin

Actin is a 43kDa filament protien that is ubiquitously expressed by all eukaryotic cells. This wide expression makes Actin an ideal "housekeeping gene", which may even account for up to 50% of total cellular protein. Actin microfilaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. alpha Actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. Actin antibodies are most commonly used as controls for protein standardization or in cytoskeletan or cell cycle research.

Alternate Names

ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, Alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3

Gene Symbol

ACTA1

UniProt

P68133 (Human)

Additional Actin Products

Product Documents for Actin Antibody (ACTA1/360)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Actin Antibody (ACTA1/360)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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