Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # ADPN/1370

Concentration

0.2 mg/ml

View all Adiponectin/Acrp30 Antibodies »

Product Summary for Adiponectin/Acrp30 Antibody (ADPN/1370)

Immunogen

Recombinant human Adiponectin/Acrp30 protein fragment (around aa 145-226) (exact sequence is proprietary) (Uniprot: Q15848)

Localization

Cytoplasmic & Cell Surface

Marker

Marker of Obesity

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54319)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Adiponectin/Acrp30 Antibody (ADPN/1370)

Western Blot: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652]

Western Blot: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652]

Western Blot: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652] - Western blot Analysis of human Kidney lysate using Adiponectin/Acrp30 Antibody (ADPN/1370).
Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652]

Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652]

Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652] - Formalin-fixed, paraffin-embedded human Kidney stained with Adiponectin Monoclonal Antibody (ADPN/1370).
Protein Array: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652]

Protein Array: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652]

Protein Array: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Adiponectin/Acrp30 Antibody (ADPN/1370). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Adiponectin/Acrp30 Antibody (ADPN/1370)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Adiponectin/Acrp30

Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Gene Symbol

ADIPOQ

UniProt

Q15848

Additional Adiponectin/Acrp30 Products

Product Documents for Adiponectin/Acrp30 Antibody (ADPN/1370)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Adiponectin/Acrp30 Antibody (ADPN/1370)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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