Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Alpha Clone # AGR 131

Concentration

1.0 mg/ml

View all Agrin Antibodies »

Product Specifications

Immunogen

Recombinant rat agrin (C-terminal fragment).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Synaptic basal lamina at the neuromuscular junction.

Specificity

detects all isoforms of agrin. This recognizes all N1 isoforms but, consistent with the epitope mapping investigations, fails to see the N4 protein. Furthermore, the will detect the X, Y and Z splice variants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2 Alpha

Scientific Data Images for Agrin Antibody (AGR 131)

Immunohistochemistry-Paraffin: Agrin Antibody (AGR 131) [NB110-11069]

Immunohistochemistry-Paraffin: Agrin Antibody (AGR 131) [NB110-11069]

Immunohistochemistry-Paraffin: Agrin Antibody (AGR 131) [NB110-11069] - Analysis of rat brain tissue.

Applications for Agrin Antibody (AGR 131)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

Western Blot

1:1000
Application Notes
Detects a band of ~100 kDa by Western blot.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Agrin

Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.

Alternate Names

AGRN

Entrez Gene IDs

11603 (Mouse); 25592 (Rat)

Gene Symbol

AGRN

UniProt

P25304

Additional Agrin Products

Product Documents for Agrin Antibody (AGR 131)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Agrin Antibody (AGR 131)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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