AIF-1/Iba1 Antibody (AIF1/8970R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24225
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Kappa Clone # AIF1/8970R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant human AIF-1/Iba1 protein (around aa 1-146) (exact sequence is proprietary)
Localization
Cytoplasm. Cell Surface.
Marker
Microglia Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Antibody without azide - store at -20 to -80C.
Scientific Data Images for AIF-1/Iba1 Antibody (AIF1/8970R) - Azide and BSA Free
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (AIF1/8970R) - Azide and BSA Free [NBP3-24225] -
Formalin-fixed, paraffin-embedded human spleen stained with AIF-1/Iba1 antibody (AIF1/8970R) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.Applications for AIF-1/Iba1 Antibody (AIF1/8970R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: AIF-1/Iba1
Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.
References
1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149
2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4
3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018
4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639
5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T., ... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316
Long Name
Allograft Inflammatory Factor 1
Alternate Names
AIF1, IBA1, IRT1
Gene Symbol
AIF1
Additional AIF-1/Iba1 Products
Product Documents for AIF-1/Iba1 Antibody (AIF1/8970R) - Azide and BSA Free
Product Specific Notices for AIF-1/Iba1 Antibody (AIF1/8970R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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