AIF-1/Iba1 Antibody

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833]

Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Pg/gingipain is present intracellularly in astrocytes, microglia and neurons in the hippocampus of experimental mice.Green: astrocytes (top panels), microglia (middle panels), and neurons (bottom panels). Red: Pg/gingipain. Blue: DAPI. Representative of N = 4 (GFAP), 3 (Iba1) and 4 (NeuN) experimental mice. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0204941), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833]

Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-2833]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-2833] - Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Pg/gingipain is present intracellularly in astrocytes, microglia & neurons in the hippocampus of experimental mice.Green: astrocytes (top panels), microglia (middle panels), & neurons (bottom panels). Red: Pg/gingipain. Blue: DAPI. Representative of N = 4 (GFAP), 3 (Iba1) & 4 (NeuN) experimental mice. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - IL-1 beta synthesis, & microglial activation modulation in PDD005-treated aged mice. (A) Images showing IL-1 beta (green fluorescence), iba-1 (red fluorescence) expression. Yellow = Overlay; Blue = nuclei; SGZ region of mouse brain. 40X magnification, scale bar = 100 μm. (B) Graphs representing the average relative fluorescence of Iba-1 in SGZ. Significant differences determined by using one-way ANOVA with Tukey’s test. *P < 0.050, **P < 0.01 & ***P < 0.001 compared with aged-vehicle. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31980673), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Lifelong choline supplementation alters the expression of the alpha7 nicotinic acetylcholine receptor ( alpha7nAchR) within microglia. (a) Photomicrographs depicting the Cornus Ammonis 1 (CA1) of the hippocampus from APP/PS1 & NonTg mice fluorescently stained for alpha7nAchR & Iba1. Images taken at 40X; scale bar = 25 µm (n = 6 mice/group). (b) Quantitative analysis reveals a significant main effect of genotype, where the APP/PS1 mice have a significantly higher intensity of yellow pixels of alpha7nAchR/Iba1 colocalization than the NonTg mice (p < .01). Additionally, we find a significant main effect of diet, where the Ch+ groups show a significant reduction in alpha7nAchR/Iba1 colocalization than the CTL groups (p < .05). A genotype by diet interaction was found, where the APP/PS1 Ch+ mice show a significant reduction of alpha7nR/Iba1 colocalization compared to the CTL counterparts (p < .001). The center line represents the median value, the limits represent the 25th & 75th percentile, & the whiskers represent the minimum & maximum value of the distribution. **p < .01, ***p < .001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31560162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - (A–D) Images of coronal brain sections showing DiI (red) labeled EVs taken up by IBA1 (blue) & CD11b (green) positive microglia. (E,F) Tomato & control plasmid (blue) dorsal SVZ electroporations stained for CD11b (green) & IBA1 (magenta) at P7, 10x (E), 20x (F). (G,H) 20x magnification of E showing CD11b (green) & IBA1 (red) (G) or CD11b (H). (I,J) Tomato & Rab27a shRNA plasmid (blue) dorsal SVZ electroporations stained for CD11b (green) & IBA1 (magenta) at P7, 10x (I), 20x (J). (K,L) 20x magnification of I showing CD11b (green) & IBA1 (red) (K) or CD11b (L). (M) Western blot of Rab27a following shRNA mediated knockdown. (N) Images of coronal section stained for Rab27a (green) following control or Rab27a shRNA & tomato (magenta) co-electroporation at P0 & sacrificed 48 hrs later. (O) Quantification of CD11b positive microglia at P2 & P7 following control or Rab27a shRNA electroporation. Data are represented as mean ± SEM. ****p < 0.0001 A, E, I scale bar, 100 μm. B–D, F–H, J–L, N scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30816224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Lifelong choline supplementation reduces activated microglia. (a) Photomicrographs depicting the Cornus Ammonis 1 (CA1) of the hippocampus from APP/PS1 & NonTg mice fluorescently stained for CD68 & Iba1. Images taken at 10X; Scale bar = 150 µm (n = 6 mice/group). Arrows illustrate colocalization of CD68/Iba1. (b) Quantitative analysis reveals a significant main effect of genotype, where the APP/PS1 mice have a significantly higher intensity of yellow pixels of CD68/Iba1 colocalization than the NonTg mice (p < .05). Additionally, we find a significant main effect of diet, where the Ch+ groups show a significant reduction in CD68/Iba1 colocalization than the CTL groups (p < .001). Data are presented as box plots. The center line represents the median value, the limits represent the 25th & 75th percentile, & the whiskers represent the minimum & maximum value of the distribution. *p < .05, ***p < .001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31560162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Lifelong choline supplementation alters the expression of the Sigma‐1 receptor ( sigma1R) within microglia. (a‐b) Representative Western blot of sigma1R levels. Quantitative analysis of sigma1R protein levels reveals a significant reduction with Ch+ (p < .05; n = 5/APP group; n = 4/NonTg group). (c‐d) Quantitative analysis reveals a significant main effect of genotype, where the APP/PS1 mice have a significantly higher intensity of yellow pixels of sigma1R/Iba1 colocalization than the NonTg mice (p < .05, n = 6 APP/PS1 CTL, n = 5 APP/PS1 Ch+, n = 6 NonTg CTL, n = 6 NonTg Ch+). Additionally, we find a significant genotype by diet interaction where the APP/PS1 Ch+ mice show a significant reduction in sigma1R/Iba1 colocalization than the APP/PS1 CTL mice (p < .001). Photomicrographs depicting the Cornus Ammonis 1 (CA1) of the hippocampus from APP/PS1 & NonTg mice fluorescently stained for the  sigma1R & Iba1; images taken at 40×; scale bar = 25 µm. Data are presented as box plots. The center line represents the median value, the limits represent the 25th & 75th percentile, & the whiskers represent the minimum & maximum value of the distribution. *p < .05, ***p < .001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31560162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: AIF-1/Iba1 Antibody [NB100-2833] -

Western Blot: AIF-1/Iba1 Antibody [NB100-2833] - Astrocytic marker was decreased in the brain of prenatally saline-exposed offspring, but not in the offspring prenatally exposed to LPS, following pharmacological activation of mGluR5.A prenatal administration of LPS did not change the brain expression level of iba1, CD68, GFAP or mGluR5 in 4-month-old mice (A). Postnatal CDPPB treatment reduced the GFAP level, without effect on iba1, CD68 or mGluR5 in the mice prenatally exposed to saline solution. The quantified molecular markers did not change in the brain of mice treated with MTEP (B). No change in iba1, CD68, GFAP or mGluR5 was observed in the brain of the mice prenatally exposed to LPS (C). Values are expressed as mean ± SEM. Abbreviations: MTEP, 3-((2-methyl-4-thiazolyl)ethynyl)pyridine; CDPPB, 3-cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl) benzamide; CD68, cluster of differentiation 68); iba1, ionized calcium binding adaptor molecule-1; GFAP, glial fibrillary acidic protein; mGluR5, metabotropic glutamate receptor subtype 5; ROD, relative optical density. **p < 0.01 >> Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26536027), licensed under a CC-BY license. Not internally tested by Novus Biologicals.