AKAP13 Antibody (5B7)

Novus Biologicals, part of Bio-Techne | Catalog # H00011214-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5B7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AKAP13 Antibodies »

Product Specifications

Immunogen

AKAP13 (NP_006729, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKLNPQQAPLYGDCVVTVLLAEEDKAEDDVVFYLVFLGSTLRHCTSTRKVSSDTLETIAPGHDCCETVKVQLCASKEGLPVFVVAEEDFHFVQDEAYDAAQFLATSAGNQ

Reactivity Notes

Human. Other species not tested.

Specificity

AKAP13 - A kinase (PRKA) anchor protein 13

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for AKAP13 Antibody (5B7)

Immunocytochemistry/ Immunofluorescence: AKAP13 Antibody (5B7) [H00011214-M01]

Immunocytochemistry/ Immunofluorescence: AKAP13 Antibody (5B7) [H00011214-M01]

Immunocytochemistry/Immunofluorescence: AKAP13 Antibody (5B7) [H00011214-M01] - Analysis of monoclonal antibody to AKAP13 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: AKAP13 Antibody (5B7) [H00011214-M01] - Detection limit for recombinant GST tagged AKAP13 is approximately 0.03ng/ml as a capture antibody.

Applications for AKAP13 Antibody (5B7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AKAP13

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in at least 3 transcript variants encoding different isoforms containing a dbl oncogene homology (DH) domain and a pleckstrin homology (PH) domain. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions. Therefore, these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway and, in addition, function as protein kinase A-anchoring proteins.

Alternate Names

A kinase (PRKA) anchor protein 13, AKAP-LbcAKAP-13, A-kinase anchor protein 13, A-kinase anchoring protein, ARHGEF13, Breast cancer nuclear receptor-binding auxiliary protein, BRXNon-oncogenic Rho GTPase-specific GTP exchange factor, c-lbc, FLJ11952, FLJ43341, Guanine nucleotide exchange factor Lbc, HA-3, HT31, Human thyroid-anchoring protein 31, LBCLBC oncogene, Lymphoid blast crisis oncogeneProtein kinase A-anchoring protein 13, p47, PROTO-LB, PROTO-LBC

Entrez Gene IDs

11214 (Human)

Gene Symbol

AKAP13

OMIM

604686 (Human)

UniProt

NP_006729 (Human)

Additional AKAP13 Products

Product Documents for AKAP13 Antibody (5B7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AKAP13 Antibody (5B7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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