ALK/CD246 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48518

Novus Biologicals, part of Bio-Techne
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NBP2-48518
NBP2-48518-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ALK/CD246 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: IFGTGHSSLESPTNMPSPSPDYFTWNLTWIMKDSFPFLSHRSRYGLECSFDFPCELEYSPPLHDLRNQSWSWRRIPSEEASQMDLLDGPGAERSKEMPRGSFLLLNTSADSKHTILS

Reactivity Notes

Mouse (84%), Rat (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ALK/CD246 Antibody

Immunocytochemistry/ Immunofluorescence: ALK/CD246 Antibody [NBP2-48518]

Immunocytochemistry/ Immunofluorescence: ALK/CD246 Antibody [NBP2-48518]

Immunocytochemistry/Immunofluorescence: ALK/CD246 Antibody [NBP2-48518] - Staining of human cell line RH-30 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ALK/CD246 Antibody [NBP2-48518]

Immunohistochemistry-Paraffin: ALK/CD246 Antibody [NBP2-48518]

Immunohistochemistry-Paraffin: ALK/CD246 Antibody [NBP2-48518] - Staining of human cerebral cortex shows cytoplasmic positivity in neurons.

Applications for ALK/CD246 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALK/CD246

ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. The 2;5 chromosomal translocation occurs in most anaplastic large-cell non-Hodgkin's lymphomas arising from activated T lymphocytes. This rearrangement was shown to fuse the NPM nucleolar phosphoprotein gene on chromosome 5q35 to a previously unidentified protein tyrosine kinase gene, ALK, on chromosome 2p23 (1). In the predicted hybrid protein, the amino terminus of NPM is linked to the catalytic domain of ALK. Immunoblotting with anti-ALK antibody shows that ALK is highly expressed in the neonatal brain (2). Also expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. Unscheduled expression of the truncated ALK may contribute to malignant transformation in these lymphomas (3).

Long Name

Anaplastic Lymphoma Kinase

Alternate Names

CD246, Ki-1, Tcrz

Gene Symbol

ALK

Additional ALK/CD246 Products

Product Documents for ALK/CD246 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ALK/CD246 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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