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Alkaline Phosphatase/ALPP Antibody (8B6) [PE/Cy5.5]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-3638PECY55

Novus Biologicals, part of Bio-Techne
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NB110-3638PECY55

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Radioimmunodiffusion, Western Blot

Label

PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 8B6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28197547). Rat reactivity reported in scientific literature (PMID: 30599898).

Localization

Cell membrane; Lipid-anchor > GPI-anchor

Specificity

Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of Alkaline Phosphatase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Applications for Alkaline Phosphatase/ALPP Antibody (8B6) [PE/Cy5.5]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Radioimmunodiffusion

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Alkaline Phosphatase/ALPP

Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.

Long Name

Alkaline Phosphatase, Placental Type

Alternate Names

Alkaline Phosphatase, PLAP, SEAP

Gene Symbol

ALPP

Additional Alkaline Phosphatase/ALPP Products

Product Documents for Alkaline Phosphatase/ALPP Antibody (8B6) [PE/Cy5.5]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Alkaline Phosphatase/ALPP Antibody (8B6) [PE/Cy5.5]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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