Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80568
![Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]](https://resources.bio-techne.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-8B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80568-img0004.jpg "Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]")
![Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6)Azide and BSA Free [NBP2-80568]](https://resources.bio-techne.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-8B6-Azide-and-BSA-Free-Western-Blot-NBP2-80568-img0001.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6)Azide and BSA Free [NBP2-80568]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Radioimmunodiffusion, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 8B6
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
Immunogen
Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Reactivity Notes
Not yet tested in other species. Mouse reactivity reported in scientific literature (PMID: 28197547). Rat reactivity reported in scientific literature (PMID: 30599898).
Localization
Cell membrane; Lipid-anchor > GPI-anchor
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6)Azide and BSA Free [NBP2-80568]
Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]](https://resources.bio-techne.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-8B6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80568-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]")
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]
Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified customer review. Image from the standard format of this antibody.![Immunohistochemistry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]](https://resources.bio-techne.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-8B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80568-img0003.jpg "Immunohistochemistry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]")
Immunohistochemistry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]
Immunohistochemistry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568] - IHC staining of Alkaline Phosphatase (Placental) in human placenta using DAB with hematoxylin counterstain. Proteolytic Induced Epitope Retrieval (PIER) was used. Image from the standard format of this antibody.Applications for Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
reported in multiple pieces of scientific literature
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:100-1:200
Western Blot
1:1000
Application Notes
In WB a band can be seen at ~70 kDa. For IHC, Proteolytic Induced Epitope Retrieval (PIER) is required. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Tris-Glycine, 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase/ALPP
Long Name
Alkaline Phosphatase, Placental Type
Alternate Names
Alkaline Phosphatase, PLAP, SEAP
Gene Symbol
ALPP
Additional Alkaline Phosphatase/ALPP Products
Product Documents for Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
Product Specific Notices for Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...