alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34577
![Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free [NBP2-34577]](https://resources.bio-techne.com/images/products/alpha-Fetoprotein-AFP-Antibody-C3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34577-img0003.jpg "Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free [NBP2-34577]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Porcine, Bovine (Negative), Canine, Monkey, Mouse (Negative), Rat (Negative)
Applications
CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # C3
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Alpha feto protein (AFP) Purified from serum of a hepatoma patient (Uniprot: P02771)
Reactivity Notes
Does not react with Bovine, Mouse or Rat.
Localization
Cytoplasmic
Specificity
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This monoclonal antibody is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
Marker
Hepatocellular/Germ Cell Tumor Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32914).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free
Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free [NBP2-34577]
Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free [NBP2-34577] - Formalin-fixed, paraffin-embedded human fetal liver stained with AFP MAb (C3).![Knockout Validated: alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free [NBP2-34577]](https://resources.bio-techne.com/images/products/alpha-Fetoprotein-AFP-Antibody-C3-Azide-and-BSA-Free-Knockout-Validated-NBP2-34577-img0004.jpg "Knockout Validated: alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free [NBP2-34577]")
Knockout Validated: alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free [NBP2-34577]
Knockout Validated: alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free [NBP2-34577] - Western blot shows lysates of HepG2 human liver carcinoma cells parental cell line and AFP knockout (KO) HepG2 cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # NBP2-32914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP at approximately 74 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.Applications for alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free
Application
Recommended Usage
ELISA
1-5ug/ml for coating
Flow Cytometry
0.5-1ug/million cells
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in Sandwich -ELISA reported in scientific literature (PMID:31921529).
Optimal dilution for a specific application should be determined.
Use in Sandwich -ELISA reported in scientific literature (PMID:31921529).
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: alpha-Fetoprotein/AFP
Alternate Names
AFP, DSCAM2
Gene Symbol
AFP
Additional alpha-Fetoprotein/AFP Products
Product Documents for alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free
Product Specific Notices for alpha-Fetoprotein/AFP Antibody (C3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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