alpha-Fetoprotein/AFP Antibody (C3) [Janelia Fluor® 549]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34577JF549
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Porcine, Bovine (Negative), Canine, Monkey, Mouse (Negative), Rat (Negative)
Applications
CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Sandwich ELISA
Label
Janelia Fluor 549
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # C3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Alpha feto protein (AFP) Purified from serum of a hepatoma patient (Uniprot: P02771)
Reactivity Notes
Does not react with Bovine, Mouse or Rat.
Localization
Cytoplasmic
Specificity
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This monoclonal antibody is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
Marker
Hepatocellular/Germ Cell Tumor Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Applications for alpha-Fetoprotein/AFP Antibody (C3) [Janelia Fluor® 549]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Knockout Validated
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: alpha-Fetoprotein/AFP
Alternate Names
AFP, DSCAM2
Gene Symbol
AFP
Additional alpha-Fetoprotein/AFP Products
Product Documents for alpha-Fetoprotein/AFP Antibody (C3) [Janelia Fluor® 549]
Product Specific Notices for alpha-Fetoprotein/AFP Antibody (C3) [Janelia Fluor® 549]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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