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Amphiphysin/AMPH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32505

Novus Biologicals, part of Bio-Techne
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NBP1-32505

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Amphiphysin/AMPH Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Amphiphysin/AMPH. The exact sequence is proprietary.

Localization

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cytoplasm, cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Amphiphysin/AMPH Antibody

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded mouse brain; antibody dilution 1:500.
Immunohistochemistry: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded U87 xenograft. Antibody at dilution of 1:500.
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.

Applications for Amphiphysin/AMPH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Amphiphysin/AMPH

Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).

Alternate Names

AMPH, AMPH1

Gene Symbol

AMPH

Additional Amphiphysin/AMPH Products

Product Documents for Amphiphysin/AMPH Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Amphiphysin/AMPH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.