Anti- Cetuximab (Anti-Idiotype) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9626
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human
Applications
ELISA, Flow Cytometry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2259C
Product Specifications
Immunogen
Full length Cetuximab
Specificity
Detects Cetuximab in Western blots and direct ELISAs. No cross-reactivity with Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Anti- Cetuximab (Anti-Idiotype) Antibody
Detection of Cetuximab by Western Blot.
Western blot shows Cetuximab, Rituximab, Brentuximab, and Gemtuzumab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Cetuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cetuximab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.Detection of anti-EGFR/ErbB1 (Cetuximab) on A431 Human Cell Line by Flow Cytometry.
A431 human epithelial carcinoma cell line was stained with anti-EGFR/ErbB1 (Cetuximab) (Catalog # MAB9577), then anti-Cetuximab (Anti-Idiotype) (Catalog # MAB9626, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).Specificity of Anti-Cetuximab (Anti-Idiotype) Antibody in Direct ELISA
A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Cetuximab (Anti-Idiotype) Antibody (Catalog # MAB9626, 333 ng/mL) detected Cetuximab. No cross-reactivity with Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.Applications for Anti- Cetuximab (Anti-Idiotype) Antibody
Application
Recommended Usage
ELISA
Detects Cetuximab in direct ELISAs.
Flow Cytometry
0.25 µg/106 cells
Sample: A431 Human cell line stained with Anti-EGFR/ErbB1 (Cetuximab), Catalog # MAB9577
Sample: A431 Human cell line stained with Anti-EGFR/ErbB1 (Cetuximab), Catalog # MAB9577
Simple Western
20 µg/mL
Sample: Cetuximab under non-reducing conditions only
Sample: Cetuximab under non-reducing conditions only
Western Blot
0.5 µg/mL
Sample: Cetuximab under non-reducing conditions only
Sample: Cetuximab under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cetuximab
Additional Cetuximab Products
Product Documents for Anti- Cetuximab (Anti-Idiotype) Antibody
Product Specific Notices for Anti- Cetuximab (Anti-Idiotype) Antibody
For research use only
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