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Anti- Cetuximab (Anti-Idiotype) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9626

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human

Applications

ELISA, Flow Cytometry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2259C

Product Specifications

Immunogen

Full length Cetuximab

Specificity

Detects Cetuximab in Western blots and direct ELISAs. No cross-reactivity with Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anti- Cetuximab (Anti-Idiotype) Antibody

Detection of Cetuximab antibody by Western Blot.

Detection of Cetuximab by Western Blot.

Western blot shows Cetuximab, Rituximab, Brentuximab, and Gemtuzumab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Cetuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cetuximab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of anti-EGFR/ErbB1 (Cetuximab) on A431 Human Cell Line antibody by Flow Cytometry.

Detection of anti-EGFR/ErbB1 (Cetuximab) on A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with anti-EGFR/ErbB1 (Cetuximab) (Catalog # MAB9577), then anti-Cetuximab (Anti-Idiotype) (Catalog # MAB9626, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).
Specificity of Anti-Cetuximab (Anti-Idiotype) Antibody in Direct Antibody in ELISA

Specificity of Anti-Cetuximab (Anti-Idiotype) Antibody in Direct ELISA

A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Cetuximab (Anti-Idiotype) Antibody (Catalog # MAB9626, 333 ng/mL) detected Cetuximab. No cross-reactivity with Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.

Applications for Anti- Cetuximab (Anti-Idiotype) Antibody

Application
Recommended Usage

ELISA

Detects Cetuximab in direct ELISAs.

Flow Cytometry

0.25 µg/106 cells
Sample: A431 Human cell line stained with Anti-EGFR/ErbB1 (Cetuximab), Catalog # MAB9577

Simple Western

20 µg/mL
Sample: Cetuximab under non-reducing conditions only

Western Blot

0.5 µg/mL
Sample: Cetuximab under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cetuximab

The anti-Cetuximab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Cetuximab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Additional Cetuximab Products

Product Documents for Anti- Cetuximab (Anti-Idiotype) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Anti- Cetuximab (Anti-Idiotype) Antibody

For research use only

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