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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Ali 12-28

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

N-terminal APC-MBP fusion protein

Localization

Cytoplasmic and Nuclear

Specificity

Ali 12-28 detects both normal and mutant-truncated forms of the APC.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for APC Antibody (Ali 12-28) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
This antibody has been used routinely for Western blotting on colon cell line HCT116, SW480 and SW837 extracts. Use in Immunohistochemistry-paraffin reported in scientific literature (Awadalla A et al).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APC

This gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

Long Name

Adenomatosis Polyposis Coli

Alternate Names

DP2, DP3, FPC, GS, Min

Entrez Gene IDs

324 (Human); 11789 (Mouse)

Gene Symbol

APC

UniProt

Additional APC Products

Product Documents for APC Antibody (Ali 12-28) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for APC Antibody (Ali 12-28) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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