APPL Antibody (4M5X9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16721

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16721-100ul
NBP3-16721-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4M5X9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all APPL Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human APPL1 (Q9UKG1). KKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPAAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for APPL Antibody (4M5X9)

Western Blot: APPL Antibody (4M5X9) [NBP3-16721]

Western Blot: APPL Antibody (4M5X9) [NBP3-16721]

Western Blot: APPL Antibody (4M5X9) [NBP3-16721] - Western blot analysis of extracts of various cell lines, using APPL1 Rabbit mAb (NBP3-16721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for APPL Antibody (4M5X9)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: APPL

APPL is encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.

Long Name

Adapter Protein Containing PH Domain PTB Domain and Leucine Zipper Motif 1

Alternate Names

APPL1, DIP13A

Gene Symbol

APPL1

Additional APPL Products

Product Documents for APPL Antibody (4M5X9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for APPL Antibody (4M5X9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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