Argonaute 3 Antibody (PCRP-AGO3-1C5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13873

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PCRP-AGO3-1C5

Concentration

0.2 mg/ml

View all Argonaute 3 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Argonaute 3 protein (Uniprot: Q9H9G7)

Localization

Cytoplasm, P-bodies, Nucleoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

97.36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14202)

Antibody with azide store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Argonaute 3 Antibody (PCRP-AGO3-1C5)

Immunocytochemistry/ Immunofluorescence: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]

Immunocytochemistry/ Immunofluorescence: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]

Immunocytochemistry/Immunofluorescence: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873] - Immunofluorescence Analysis of PFA-fixed HeLa cells. Cytoplasmic P-bodies and nucleoplasm stained using Argonaute 3 Antibody (PCRP-AGO3-1C5) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]

Flow Cytometry: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]

Flow Cytometry: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873] - Flow cytometric analysis of PFA-fixed HeLa cells. Argonaute 3 antibody (PCRP-AGO3-1C5) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]

Protein Array: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]

Protein Array: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Argonaute 3 Antibody (PCRP-AGO3-1C5).

Applications for Argonaute 3 Antibody (PCRP-AGO3-1C5)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Argonaute 3

Argonaute 3 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

AGO3argonaute 3, Argonaute3, eIF2C 3, eIF-2C 3, Eukaryotic translation initiation factor 2C 3, eukaryotic translation initiation factor 2C, 3, FLJ12765, hAgo3, MGC86946, protein argonaute-3

Gene Symbol

AGO3

UniProt

Q9H9G7 (Human)

Additional Argonaute 3 Products

Product Documents for Argonaute 3 Antibody (PCRP-AGO3-1C5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Argonaute 3 Antibody (PCRP-AGO3-1C5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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