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Argonaute 3 Antibody (PCRP-AGO3-1C5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14202

Novus Biologicals, part of Bio-Techne
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NBP3-14202

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PCRP-AGO3-1C5

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human Argonaute 3 protein (Uniprot: Q9H9G7)

Localization

Cytoplasm, P-bodies, Nucleoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

97.36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13873).

Antibody with azide store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Argonaute 3 Antibody (PCRP-AGO3-1C5) - Azide and BSA Free

Protein Array: Argonaute 3 Antibody (PCRP-AGO3-1C5) - Azide and BSA Free [NBP3-14202]

Protein Array: Argonaute 3 Antibody (PCRP-AGO3-1C5) - Azide and BSA Free [NBP3-14202]

Protein Array: Argonaute 3 Antibody (PCRP-AGO3-1C5) - Azide and BSA Free [NBP3-14202] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Argonaute 3 Antibody (PCRP-AGO3-1C5).

Applications for Argonaute 3 Antibody (PCRP-AGO3-1C5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Argonaute 3

Argonaute 3 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

AGO3argonaute 3, Argonaute3, eIF2C 3, eIF-2C 3, Eukaryotic translation initiation factor 2C 3, eukaryotic translation initiation factor 2C, 3, FLJ12765, hAgo3, MGC86946, protein argonaute-3

Gene Symbol

AGO3

Additional Argonaute 3 Products

Product Documents for Argonaute 3 Antibody (PCRP-AGO3-1C5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Argonaute 3 Antibody (PCRP-AGO3-1C5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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