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ASGPR1 Antibody (021)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89636

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-89636-100ul
NBP2-89636-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 021

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for ASGPR1 Antibody (021)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ASGPR1 (Uniprot#: P07306; Gln62-Leu291).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ASGPR1 Antibody (021)

Western Blot: ASGPR1 Antibody (021) [NBP2-89636]

Western Blot: ASGPR1 Antibody (021) [NBP2-89636]

Western Blot: ASGPR1 Antibody (021) [NBP2-89636] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDa Observed band size:43 kDa
Immunoprecipitation: ASGPR1 Antibody (021) [NBP2-89636]

Immunoprecipitation: ASGPR1 Antibody (021) [NBP2-89636]

Immunoprecipitation: ASGPR1 Antibody (021) [NBP2-89636] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-ASGR1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-ASGR1 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa Observed band size: 50 kDa

Applications for ASGPR1 Antibody (021)

Application
Recommended Usage

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Application Notes
This antibody will detect Human ASGPR1 in ELISA pair set (Catalog: # NBP2-79552). In a sandwich ELISA, it can be used as a capture antibody when paired with (Catalog: # NBP2-89638).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASGR1/ASGPR1

Partially deglycosylated plasma glycoproteins and immunoglobulin IgA2 allotypes are efficiently and specificallyremoved from circulation by a receptor-mediated process. The asialoglycoprotein receptor binds to desialylated(galactosyl-terminal) glycoproteins. It transports these glycoproteins via a series of membrane vesicles and tubulesto an acidic-sorting organelle where the receptor and ligand dissociate. Then the receptor is recycled back to thecell surface and the ligand is transported to the lysosomes for degradation. (provided by RefSeq)

Long Name

Asialoglycoprotein Receptor 1

Alternate Names

ASGPR1, CLEC4H1, MHL1, RHL1

Gene Symbol

ASGR1

Additional ASGR1/ASGPR1 Products

Product Documents for ASGPR1 Antibody (021)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ASGPR1 Antibody (021)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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