ATM [p Ser1981] Antibody (10H11.E12) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-306B

Submit a review

Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NB100-306

Alexa Fluor 350

NB100-306AF350

Alexa Fluor 405

NB100-306AF405

Alexa Fluor 488

NB100-306AF488

Alexa Fluor 532

NB100-306AF532

Alexa Fluor 594

NB100-306AF594

Alexa Fluor 647

NB100-306AF647

Alexa Fluor 700

NB100-306AF700

Alexa Fluor 750

NB100-306AF750

CoraFluor 1

NB100-306CL1

DyLight 350

NB100-306UV

DyLight 405

NB100-306V

DyLight 488

NB100-306G

DyLight 550

NB100-306R

DyLight 594

NB100-306DL594

DyLight 650

NB100-306C

DyLight 680

NB100-306FR

DyLight 755

NB100-306IR

FITC

NB100-306F

HRP

NB100-306H

Janelia Fluor 525

NB100-306JF525

Janelia Fluor 549

NB100-306JF549

Janelia Fluor 646

NB100-306JF646

Janelia Fluor 669

NB100-306JF669

mFluor Violet 450 SE

NB100-306MFV450

mFluor Violet 500 SE

NB100-306MFV500

mFluor Violet 610 SE

NB100-306MFV610

Forumulation

Catalog #

Azide and BSA Free

NBP2-80586

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine (Negative)

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 10H11.E12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATM Antibodies »

Product Specifications

Immunogen

ATM [p Ser1981] Antibody (10H11.E12) [Biotin] was made to a synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM. [UniProt# Q13315]

Modification

p Ser1981

Localization

Nucleus, cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Theoretical MW

351 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for ATM [p Ser1981] Antibody (10H11.E12) [Biotin]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: ATM

ATM (ataxia telangiectasia mutated kinase) is the master regulator of the DNA double-strand break (DSB) repair pathway. This ubiquitously expressed serine/threonine protein kinase belongs to the PI3K-like family of proteins and responds to DSBs caused by oxidative and other genotoxic stresses (1). In addition to regulating the DNA damage response, ATM participates in vesicle and protein transport, T-cell development, gonads/neurological function, pre-B cell allelic exclusion, cell cycle control, and acts as a tumor suppressor (2,3). Defects in ATM are associated with ataxia telangiectasia (AT), T-cell acute lymphoblastic leukemia (TALL), T-prolymphocytic leukemia (TPLL), and B-cell non-Hodgkin lymphomas (BNHL) including mantle cell lymphoma (MCL) and B-cell chronic lymphocytic leukemia (BCLL) (4).

The theoretical molecular weight of ATM is 350 kDa and it has 3 main domains: a FAT (focal adhesion targeting) domain (aa 1960-2566), a PI-3/PI-4 kinase catalytic domain (aa 2712-2962), and a C-terminal FAT domain (aa 3024-3056). ATM exists as a dimer or tetramer in its inactive state. Upon sensing DNA damage, the MRE11-RAD50-NBS1 (MRN) complex recruits ATM. The intricate process of ATM activation involves acetylation by KAT5/TIP60, autophosphorylation at Ser-1981, and dissociation into catalytically active monomers (5). Following activation, ATM phosphorylates multiple substrates such as p53/TP53 and Chk2 involved in DNA repair, checkpoint signaling, and the apoptosis pathway.

References

1. Paull TT. (2015) Mechanisms of ATM Activation. Annu Rev Biochem. 84:711-38. PMID: 25580527

2. Chaudhary MW and Al-Baradie RS. (2014) Ataxia-telangiectasia: future prospects. Appl Clin Genet. 7:159-167. PMID: 25258552

3. Stagni V, Cirotti C, and Barila D. (2018) Ataxia-Telangiectasia Mutated Kinase in the Control of Oxidative Stress, Mitochondria, and Autophagy in Cancer: A Maestro With a Large Orchestra. Front Oncol. 8:73. PMID: 29616191

4. Gumy-Pause F, Wacker P, and Sappino AP. (2004) ATM gene and lymphoid malignancies. Leukemia. 18(2):238-42. PMID: 14628072

5. Adamowicz M. (2018) Breaking up with ATM. J Immunol Sci. 2(1):26-31. PMID: 29652413

Long Name

Ataxia Telangiectasia-mutated

Alternate Names

TEL1, TELO1, TPLL

Gene Symbol

ATM

Additional ATM Products

Product Documents for ATM [p Ser1981] Antibody (10H11.E12) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for ATM [p Ser1981] Antibody (10H11.E12) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews