ATM Antibody (4E11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26493
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4E11
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ATM Antibody (4E11)
Immunogen
A synthesized peptide derived from Human ATM [UniProt Q13315]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ATM Antibody (4E11)
Immunoprecipitation: ATM Antibody (4E11) [NBP3-26493] -
Immunoprecipitation: ATM Antibody (4E11) [NBP3-26493] - Immunoprecipitating ATM in PC3 whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26493 in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26493 (3ug) + PC3 whole cell lysate (500ug).
Lane 3: PC3 whole cell lysate (20ug).
Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] -
Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] - Image of ATM Antibody (4E11) diluted at 1:205 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] -
Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] - Image of ATM Antibody (4E11) diluted at 1:205 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.Applications for ATM Antibody (4E11)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: ATM
The theoretical molecular weight of ATM is 350 kDa and it has 3 main domains: a FAT (focal adhesion targeting) domain (aa 1960-2566), a PI-3/PI-4 kinase catalytic domain (aa 2712-2962), and a C-terminal FAT domain (aa 3024-3056). ATM exists as a dimer or tetramer in its inactive state. Upon sensing DNA damage, the MRE11-RAD50-NBS1 (MRN) complex recruits ATM. The intricate process of ATM activation involves acetylation by KAT5/TIP60, autophosphorylation at Ser-1981, and dissociation into catalytically active monomers (5). Following activation, ATM phosphorylates multiple substrates such as p53/TP53 and Chk2 involved in DNA repair, checkpoint signaling, and the apoptosis pathway.
References
1. Paull TT. (2015) Mechanisms of ATM Activation. Annu Rev Biochem. 84:711-38. PMID: 25580527
2. Chaudhary MW and Al-Baradie RS. (2014) Ataxia-telangiectasia: future prospects. Appl Clin Genet. 7:159-167. PMID: 25258552
3. Stagni V, Cirotti C, and Barila D. (2018) Ataxia-Telangiectasia Mutated Kinase in the Control of Oxidative Stress, Mitochondria, and Autophagy in Cancer: A Maestro With a Large Orchestra. Front Oncol. 8:73. PMID: 29616191
4. Gumy-Pause F, Wacker P, and Sappino AP. (2004) ATM gene and lymphoid malignancies. Leukemia. 18(2):238-42. PMID: 14628072
5. Adamowicz M. (2018) Breaking up with ATM. J Immunol Sci. 2(1):26-31. PMID: 29652413
Long Name
Ataxia Telangiectasia-mutated
Alternate Names
TEL1, TELO1, TPLL
Gene Symbol
ATM
Additional ATM Products
Product Documents for ATM Antibody (4E11)
Product Specific Notices for ATM Antibody (4E11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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