ATOX1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06611

Novus Biologicals, part of Bio-Techne
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NBP1-06611
NBP1-06611SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all ATOX1 Antibodies »

Product Specifications

Immunogen

Full length human ATOX1 protein [Swiss-Prot# O00244]

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATOX1 Antibody

Western Blot: ATOX1 Antibody [NBP1-06611]

Western Blot: ATOX1 Antibody [NBP1-06611]

Western Blot: ATOX1 Antibody [NBP1-06611] - HeLa whole cell extract.
Immunocytochemistry/ Immunofluorescence: ATOX1 Antibody [NBP1-06611]

Immunocytochemistry/ Immunofluorescence: ATOX1 Antibody [NBP1-06611]

Immunocytochemistry/Immunofluorescence: ATOX1 Antibody [NBP1-06611] - ATOX1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Applications for ATOX1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Western Blot

1:1000
Application Notes
This ATOX1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis. In Western blot a band is seen at ~6 kDa representing ATOX1 protein. In Immunocytochemistry/Immunofluorescence cytoplasmic staining is observed in HeLa cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATOX1

ATOX1 (anti-oxidant 1 or HAH1) is an ATX1 family member and is a ubiquitously expressed metallochaperone which binds/deliver cytosolic copper to copper ATPase proteins. Copper is an essential trace element which at high concentration is extremely toxic and a balance between copper import/export is highly critical for survival. To ensure that the total pool of free copper is less than 1 ion per cell, ATOX1 specifically deliver copper to cellular compartments and copper-dependent enzymes. ATOX1 interacts with ATP7A (Menkes protein) and ATP7B (Wilson protein) in a copper-dependent manner leading to facilitation of copper transfer from ATOX1 to ATP7A or ATP7B, thus permitting copper transport into the secretory pathway and copper export from the cell. The conserved metal binding site of ATOX1, CxGC, differs from the metal-binding sites of Cu-transporting ATPases and has a physiologically relevant redox potential that equilibrates with the GSH: GSSH pair. Cellular functions of ATOX1 are not limited to its copper-trafficking role and may include storage of labile copper, modulation of transcription, and antioxidant defense.

Alternate Names

ATX1, ATX1 (antioxidant protein 1, yeast) homolog 1, ATX1 antioxidant protein 1 homolog (yeast), copper transport protein ATOX1, hah1, HAH1 MGC138453, Metal transport protein ATX1, MGC138455

Entrez Gene IDs

475 (Human)

Gene Symbol

ATOX1

UniProt

O00244 (Human)

Additional ATOX1 Products

Product Documents for ATOX1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATOX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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