B-Raf Antibody (OTI5A9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70260

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5A9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all B-Raf Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

84.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for B-Raf Antibody (OTI5A9) - Azide and BSA Free

Western Blot: B-Raf Antibody (OTI5A9)Azide and BSA Free [NBP2-70260]

Western Blot: B-Raf Antibody (OTI5A9)Azide and BSA Free [NBP2-70260]

Western Blot: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antibody
Immunocytochemistry/ Immunofluorescence: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260]

Immunocytochemistry/ Immunofluorescence: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260]

Immunocytochemistry/Immunofluorescence: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY B Raf.
Immunohistochemistry: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260]

Immunohistochemistry: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260]

Immunohistochemistry: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - Staining of paraffin-embedded Human pancreas tissue using anti-B Raf mouse monoclonal antibody.
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Applications for B-Raf Antibody (OTI5A9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf Antibody (OTI5A9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B-Raf Antibody (OTI5A9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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