Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all B-Raf Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid peptide near the center of human B-raf. The immunogen is located within amino acids 330 - 380 of B-raf. Amino Acid Squence: QPFRPADEDHRNQFGQRDR

Specificity

Human B-raf has one isoform (766aa, 84kD). Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD). Rat B-raf a has one isoform (712aa, 80kD). NBP2-41219 can detect in human B-raf and mouse isoform 1. This antibody will not cross-react with C-raf.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for B-Raf Antibody - BSA Free

Western Blot: B-Raf AntibodyBSA Free [NBP2-41219]

Western Blot: B-Raf AntibodyBSA Free [NBP2-41219]

Western Blot: B-Raf Antibody [NBP2-41219] - 15 ug of lysates per lane. Antibodies: B-raf 5777, (0.5 ug/mL), B-raf 7379, (0.5 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antichicken and goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
B-Raf Antibody - BSA Free

Immunohistochemistry: B-Raf Antibody - BSA Free [NBP2-41219] -

Immunohistochemistry: B-Raf Antibody - BSA Free [NBP2-41219] - Validation of B-Raf in Human Small Intestine Tissue. Immunohistochemical analysis of paraffin-embedded Human Small Intestine Tissue using anti-B-Raf antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western Blot: B-Raf AntibodyBSA Free [NBP2-41219]

Western Blot: B-Raf AntibodyBSA Free [NBP2-41219]

Western Blot: B-Raf Antibody [NBP2-41219] - Analysis of B-raf in human brain tissue lysate with B-raf antibody at (A) 1 and (B) 2 ug/mL.
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Applications for B-Raf Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

0.5- 2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B-Raf

B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B-Raf Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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