B220/CD45R Antibody (PTPRC/1783R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53303
Recombinant Monoclonal Antibody.
![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Flow-Cytometry-NBP2-53303-img0005.jpg "Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
![Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Western-Blot-NBP2-53303-img0006.jpg "Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PTPRC/1783R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human full-length B220/CD45R protein (Uniprot: P08575 )
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:31094704).
Localization
Cell Surface and Cytoplasmic
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
200ug/ml of antibody purified by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54578)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for B220/CD45R Antibody (PTPRC/1783R)
Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]
Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Western Blot Analysis of human spleen tissue lysate using B220/CD45R antibody (PTPRC/1783R).![Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Immunocytochemistry-Immunofluorescence-NBP2-53303-img0003.jpg "Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]
Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Paraformaldehyde-fixed Raji cells stained with B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Green). Counterstain is Phalloidin (red).![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Immunohistochemistry-Paraffin-NBP2-53303-img0004.jpg "Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - FFPE section of human lung cancer. 10X magnification. IHC-P image submitted by a verified customer review.![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Immunohistochemistry-Paraffin-NBP2-53303-img0001.jpg "Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Flow-Cytometry-NBP2-53303-img0002.jpg "Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Applications for B220/CD45R Antibody (PTPRC/1783R)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NBP2-53303 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: B220/CD45R
Additional B220/CD45R Products
Product Documents for B220/CD45R Antibody (PTPRC/1783R)
Product Specific Notices for B220/CD45R Antibody (PTPRC/1783R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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