B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54578
Recombinant Monoclonal Antibody.
![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free [NBP2-54578]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54578-img0004.jpg "Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free [NBP2-54578]")
![Western Blot: B220/CD45R Antibody (PTPRC/1783R)Azide and BSA Free [NBP2-54578]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Azide-and-BSA-Free-Western-Blot-NBP2-54578-img0005.jpg "Western Blot: B220/CD45R Antibody (PTPRC/1783R)Azide and BSA Free [NBP2-54578]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PTPRC/1783R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human full-length B220/CD45R protein (Uniprot: P08575 )
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:31094704).
Localization
Cell Surface and Cytoplasmic
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified by Protein A. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53303).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free
Western Blot: B220/CD45R Antibody (PTPRC/1783R)Azide and BSA Free [NBP2-54578]
Western Blot: B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free [NBP2-54578] - Western Blot Analysis of human spleen tissue lysate using B220/CD45R antibody (PTPRC/1783R).![Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free [NBP2-54578]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54578-img0003.jpg "Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free [NBP2-54578]")
Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free [NBP2-54578]
Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free [NBP2-54578] - Paraformaldehyde-fixed Raji cells stained with B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Green). Counterstain is Phalloidin (red).![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free [NBP2-54578]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54578-img0001.jpg "Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free [NBP2-54578]")
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free [NBP2-54578]
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free [NBP2-54578] - Human Tonsil stained with CD45RB Recombinant Rabbit Monoclonal Antibody (PTPRC/1783R).Applications for B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5 - 1 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: B220/CD45R
Long Name
Cluster of Differentiation 45
Alternate Names
CD45R
Gene Symbol
PTPRC
Additional B220/CD45R Products
Product Documents for B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free
Product Specific Notices for B220/CD45R Antibody (PTPRC/1783R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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