B220/CD45R Antibody (YKIX753.22.2)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65018

Novus Biologicals, part of Bio-Techne
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NB100-65018-0.1mg
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Key Product Details

Species Reactivity

Canine

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # YKIX753.22.2

Concentration

1.0 mg/ml

View all B220/CD45R Antibodies »

Product Specifications

Immunogen

Canine thymocytes

Specificity

This antibody recognizes canine CD45R and was clustered as a canine CD45R at the first canine Leucocyte Antigen workshop. It precipitates an antigen of 220kD from Con A Blasts (1) and may be against a CD45R isoform. It is expressed on subsets of leucocytes (neutrophils are weak) in canine blood of all out bred mongrels and beagles tested.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for B220/CD45R Antibody (YKIX753.22.2)

Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]

Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]

Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) [NB100-65018] - Figure A. RPE conjugated Rat anti Canine CD45R and Alexa Fluor 488 conjugated Rat IgG1 isotype control. Figure B. RPE conjugated Mouse anti Canine CD45R and Alexa Fluor 488 conjugated Rat anti Canine CD44.
Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]

Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]

Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) [NB100-65018] - Figure A. Alexa Fluor 488 conjugated Rat anti Dog CD44 and Alexa Fluor 647 conjugated Rat IgG2b isotype control. Figure B. Alexa Fluor 488 conjugated Rat anti Dog CD44 and Alexa Fluor 647 conjugated Rat anti Dog CD45R.
Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]

Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]

Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) [NB100-65018] - Figure A. Alexa Fluor 488 conjugated Rat anti Canine CD44 and RPE conjugated Rat IgG2a isotype control. Figure B. Alexa Fluor 488 conjugated Rat anti Canine CD44 and RPE conjugated Rat anti Canine CD45R.

Applications for B220/CD45R Antibody (YKIX753.22.2)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B220/CD45R

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognizing a common epitope on all of the isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Long Name

Cluster of Differentiation 45

Alternate Names

CD45R

Gene Symbol

PTPRC

Additional B220/CD45R Products

Product Documents for B220/CD45R Antibody (YKIX753.22.2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for B220/CD45R Antibody (YKIX753.22.2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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