B220/CD45R Antibody (YKIX753.22.2)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65018
![Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-YKIX753-22-2-Flow-Cytometry-NB100-65018-img0003.jpg "Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Canine
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # YKIX753.22.2
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Canine thymocytes
Reactivity Notes
Reacts with Canine.
Specificity
This antibody recognizes canine CD45R and was clustered as a canine CD45R at the first canine Leucocyte Antigen workshop. It precipitates an antigen of 220kD from Con A Blasts (1) and may be against a CD45R isoform. It is expressed on subsets of leucocytes (neutrophils are weak) in canine blood of all out bred mongrels and beagles tested.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for B220/CD45R Antibody (YKIX753.22.2)
Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]
Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) [NB100-65018] - Figure A. RPE conjugated Rat anti Canine CD45R and Alexa Fluor 488 conjugated Rat IgG1 isotype control. Figure B. RPE conjugated Mouse anti Canine CD45R and Alexa Fluor 488 conjugated Rat anti Canine CD44.![Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-YKIX753-22-2-Flow-Cytometry-NB100-65018-img0001.jpg "Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]")
Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]
Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) [NB100-65018] - Figure A. Alexa Fluor 488 conjugated Rat anti Dog CD44 and Alexa Fluor 647 conjugated Rat IgG2b isotype control. Figure B. Alexa Fluor 488 conjugated Rat anti Dog CD44 and Alexa Fluor 647 conjugated Rat anti Dog CD45R.![Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-YKIX753-22-2-Flow-Cytometry-NB100-65018-img0002.jpg "Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]")
Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) - BSA Free [NB100-65018]
Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) [NB100-65018] - Figure A. Alexa Fluor 488 conjugated Rat anti Canine CD44 and RPE conjugated Rat IgG2a isotype control. Figure B. Alexa Fluor 488 conjugated Rat anti Canine CD44 and RPE conjugated Rat anti Canine CD45R.Applications for B220/CD45R Antibody (YKIX753.22.2)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: B220/CD45R
Long Name
Cluster of Differentiation 45
Alternate Names
CD45R
Gene Symbol
PTPRC
Additional B220/CD45R Products
Product Documents for B220/CD45R Antibody (YKIX753.22.2)
Product Specific Notices for B220/CD45R Antibody (YKIX753.22.2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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