B220/CD45R Antibody (YKIX753.22.2) [Allophycocyanin/Cy7]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65018APCCY7
Conjugate
Catalog #
Key Product Details
Species Reactivity
Canine
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Allophycocyanin/Cy7 (Excitation = 650;755 nm, Emission = 767 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # YKIX753.22.2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Canine thymocytes
Reactivity Notes
Reacts with Canine.
Specificity
This antibody recognizes canine CD45R and was clustered as a canine CD45R at the first canine Leucocyte Antigen workshop. It precipitates an antigen of 220kD from Con A Blasts (1) and may be against a CD45R isoform. It is expressed on subsets of leucocytes (neutrophils are weak) in canine blood of all out bred mongrels and beagles tested.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for B220/CD45R Antibody (YKIX753.22.2) [Allophycocyanin/Cy7]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: B220/CD45R
Long Name
Cluster of Differentiation 45
Alternate Names
CD45R
Gene Symbol
PTPRC
Additional B220/CD45R Products
Product Documents for B220/CD45R Antibody (YKIX753.22.2) [Allophycocyanin/Cy7]
Product Specific Notices for B220/CD45R Antibody (YKIX753.22.2) [Allophycocyanin/Cy7]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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