B7-1/CD80 Antibody (2D10.4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43384
Clone 2D10.4 was used by HLDA to establish CD designation.
![Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-2D10-4-Flow-Cytometry-NBP1-43384-img0006.jpg "Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Functional
Cited:
Flow Cytometry, Functional Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2D10.4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen for this antibody was CD80.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for B7-1/CD80 Antibody (2D10.4)
Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384]
Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE/Cy5 conjugate of NBP1-43384. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 kappa Isotype Control (open histogram) orAnti-Human CD80 (B7-1) PE-Cy5 (filled histogram).![Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-2D10-4-Flow-Cytometry-NBP1-43384-img0004.jpg "Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384]")
Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384]
Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the FITC conjugate of NBP1-43384. Staining of the Daudi cell line with Mouse IgG1 kappa Isotype Control FITC (cat. 11-4714) (blue histogram) or Anti-Human CD80 (B7-1) FITC (purple histogram). Total viable cells were used for analysis.![Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-2D10-4-Flow-Cytometry-NBP1-43384-img0005.jpg "Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384]")
Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384]
Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE conjugate of NBP1-43384. Analysis of human peripheral blood leukocytes using CD80 PE conjugage. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.Applications for B7-1/CD80 Antibody (2D10.4)
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Application Notes
The 2D10.4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in functional reported in scientific literature (PMID 15951837)
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: B7-1/CD80
Alternate Names
B71, CD80
Gene Symbol
CD80
Additional B7-1/CD80 Products
Product Documents for B7-1/CD80 Antibody (2D10.4)
Product Specific Notices for B7-1/CD80 Antibody (2D10.4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...