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B7-1/CD80 Antibody (2D10.4) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43616

Clone 2D10.4 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP1-43616-0.1mg

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D10.4

Concentration

5 mg/ml

Product Specifications

Immunogen

The immunogen for this antibody was CD80.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for B7-1/CD80 Antibody (2D10.4) [Biotin]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
The 2D10.4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: B7-1/CD80

The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Alternate Names

B71, CD80

Gene Symbol

CD80

Additional B7-1/CD80 Products

Product Documents for B7-1/CD80 Antibody (2D10.4) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for B7-1/CD80 Antibody (2D10.4) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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