B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80588

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 62N3G8

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all B7-1/CD80 Antibodies »

Product Summary for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

Immunogen

Amino acids 102-223 of human CD80 were used as the immunogen for the antibody.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27440778).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]

Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]

Western Blot: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis of CD80 in A) a partial recombinant protein and B) Daudi lysate using this antibody at 0.1 and 1 g/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.
Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]

Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]

Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining. Image from the standard format of this antibody.
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]

Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]

Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25255. Staining of CD80 in human PBMC (monocyte depleted) using anti-CD80 antibody. Image from verified customer review. Image from the standard format of this antibody.
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Applications for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000. Use reported by customer review

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

reported by customer review
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B7-1/CD80

The CD80 antigen (B7-1, BB1) recognizes a 60-kD transmembrane glycoprotein that is a member of the immunoglobulin family. This molecule shares with CD86 the capability to be the ligand for two structurally similar molecules expressed on T lymphocytes, CD28 and CD152 (CTLA-4). CD80 antigen is expressed on in vitro activated B lymphocytes after 24h stimulation and at maximal level 48-72h post activation. It is not expressed on the majority of resting B cells from peripheral blood but identifies a subpopulation of B cells that has been previously activated. The antigen is also expressed by HTLV-1 transformed T cells activated monocytes, and constitutively on dendritic cells. CD80 binding provides costimulatory signals for T cell activation.

Alternate Names

B71, CD80

Gene Symbol

CD80

Additional B7-1/CD80 Products

Product Documents for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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