B7-1/CD80 Antibody (RM80) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11976

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Product Datasheet / COA

Recombinant Monoclonal Antibody

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP3-11976AF350

Alexa Fluor 405

NBP3-11976AF405

Alexa Fluor 488

NBP3-11976AF488

Alexa Fluor 532

NBP3-11976AF532

Alexa Fluor 594

NBP3-11976AF594

Alexa Fluor 647

NBP3-11976AF647

Alexa Fluor 700

NBP3-11976AF700

Alexa Fluor 750

NBP3-11976AF750

Allophycocyanin

NBP3-11976APC

Biotin

NBP3-11976B

CoraFluor 1

NBP3-11976CL1

DyLight 350

NBP3-11976UV

DyLight 405

NBP3-11976V

DyLight 488

NBP3-11976G

DyLight 550

NBP3-11976R

DyLight 594

NBP3-11976DL594

DyLight 650

NBP3-11976C

DyLight 680

NBP3-11976FR

DyLight 755

NBP3-11976IR

FITC

NBP3-11976F

HRP

NBP3-11976H

Janelia Fluor 525

NBP3-11976JF525

Janelia Fluor 549

NBP3-11976JF549

Janelia Fluor 585

NBP3-11976JF585

Janelia Fluor 635

NBP3-11976JF635

Janelia Fluor 646

NBP3-11976JF646

Janelia Fluor 669

NBP3-11976JF669

mFluor Violet 450 SE

NBP3-11976MFV450

mFluor Violet 500 SE

NBP3-11976MFV500

mFluor Violet 610 SE

NBP3-11976MFV610

PE

NBP3-11976PE

PerCP

NBP3-11976PCP

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Block/Neutralize, Flow Cytometry, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # RM80

Format

Azide and BSA Free

Concentration

1 mg/ml

View all B7-1/CD80 Antibodies »

Product Summary for B7-1/CD80 Antibody (RM80) - Chimeric - Azide and BSA Free

Immunogen

This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.

Specificity

This antibody is specific for murine B7-1/CD80, a ~55 kDa member of the Ig superfamily. B7-1/CD80 is expressed by macrophages, dendritic cells, activated B cells, and activated T cells. B7-1/CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with B7-1/CD80 is crucial in T-B cell communication leading to the activation of T and B cells, respectively.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for B7-1/CD80 Antibody (RM80) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: B7-1/CD80 Antibody (RM80) - Chimeric - Azide and BSA Free [NBP3-11976]

Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (RM80) - Chimeric [NBP3-11976] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RM80 (NBP3-11976) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11976, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for B7-1/CD80 Antibody (RM80) - Chimeric - Azide and BSA Free

Application

Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional Assay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: B7-1/CD80

The B-lymphocyte activation antigen B7-1 (CD80) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines (1). The relatively small CTLA-4/B7-1 binding interface exhibits an unusually high degree of shape complementarity. CTLA-4 forms homodimers through a newly defined interface of highly conserved residues. This zipper-like oligomerization provides the structural basis for forming unusually stable signalling complexes at the T-cell surface, underscoring the importance of potent inhibitory signalling in human immune responses (2).

Alternate Names

B71, CD80

Gene Symbol

CD80

Additional B7-1/CD80 Products

Product Documents for B7-1/CD80 Antibody (RM80) - Chimeric - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for B7-1/CD80 Antibody (RM80) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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