BACE-1 Antibody (7C5T7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15398

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15398-100ul
NBP3-15398-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7C5T7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BACE-1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 402-501 of human BACE-1 (P56817). FYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRQQHDDFADDISLLK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BACE-1 Antibody (7C5T7)

Western Blot: BACE-1 Antibody (7C5T7) [NBP3-15398]

Western Blot: BACE-1 Antibody (7C5T7) [NBP3-15398]

Western Blot: BACE-1 Antibody (7C5T7) [NBP3-15398] - Western blot analysis of extracts of various cell lines, using BACE-1 Rabbit mAb (NBP3-15398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: BACE-1 Antibody (7C5T7) [NBP3-15398]

Western Blot: BACE-1 Antibody (7C5T7) [NBP3-15398]

Western Blot: BACE-1 Antibody (7C5T7) [NBP3-15398] - Western blot analysis of extracts of SH-SY5Y cells, using BACE-1 Rabbit mAb (NBP3-15398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for BACE-1 Antibody (7C5T7)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BACE-1

BACE1 is a protease responsible for the proteolytic processing of the amyloid precursor protein (APP). Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 1

Alternate Names

BACE1

Gene Symbol

BACE1

Additional BACE-1 Products

Product Documents for BACE-1 Antibody (7C5T7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BACE-1 Antibody (7C5T7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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