Bcl-10 Antibody (OTI4A8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71398

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4A8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Bcl-10 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BCL10 (NP_003912) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

26.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bcl-10 Antibody (OTI4A8) - Azide and BSA Free

Western Blot: Bcl-10 Antibody (OTI4A8)Azide and BSA Free [NBP2-71398]

Western Blot: Bcl-10 Antibody (OTI4A8)Azide and BSA Free [NBP2-71398]

Western Blot: Bcl-10 Antibody (OTI4A8) - Azide and BSA Free [NBP2-71398] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Bcl10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Bcl10.
Immunocytochemistry/ Immunofluorescence: Bcl-10 Antibody (OTI4A8) - Azide and BSA Free [NBP2-71398]

Immunocytochemistry/ Immunofluorescence: Bcl-10 Antibody (OTI4A8) - Azide and BSA Free [NBP2-71398]

Immunocytochemistry/Immunofluorescence: Bcl-10 Antibody (OTI4A8) - Azide and BSA Free [NBP2-71398] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Bcl10.
Immunohistochemistry: Bcl-10 Antibody (OTI4A8) - Azide and BSA Free [NBP2-71398]

Immunohistochemistry: Bcl-10 Antibody (OTI4A8) - Azide and BSA Free [NBP2-71398]

Immunohistochemistry: Bcl-10 Antibody (OTI4A8) - Azide and BSA Free [NBP2-71398] - Staining of paraffin-embedded Human liver tissue using anti-Bcl10 mouse monoclonal antibody.
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Applications for Bcl-10 Antibody (OTI4A8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl-10

Bcl-10 is an adaptor protein with a caspase recruitment domain (CARD) that promotes apoptosis and activates pro-caspase 9 and NF-kB (1-2). Bcl-10 also interacts with other CARD domain containing proteins including CARD9, 10, 11 and 14. Bcl-10 is found to form a complex with MALT1, a protein known to be translocated in MALT lymphomas. Bcl-10 and MALT1 play a critical role in antigen receptor

Long Name

B Cell Lymphoma/Leukemia 10

Alternate Names

Bcl10, c-E10, CARMEN, CIPER, CLAP, mE10

Gene Symbol

BCL10

Additional Bcl-10 Products

Product Documents for Bcl-10 Antibody (OTI4A8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Bcl-10 Antibody (OTI4A8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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