Bcl-6 Antibody (BCL6/1982) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75749CL1
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BCL6/1982
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Bcl-6 Antibody (BCL6/1982) [CoraFluor™ 1]
Immunogen
Recombinant human bcl-6 protein fragment (around aa256-389) (exact sequence is proprietary) (Uniprot: P41182)
Localization
Nuclear
Specificity
Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ('LH') cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
Marker
Follicular Lymphoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Bcl-6 Antibody (BCL6/1982) [CoraFluor™ 1]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Protein Array
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Bcl-6
Long Name
B Cell Lymphoma/Leukemia 6
Alternate Names
Bcl-5, Bcl6, LAZ3, ZBTB27, ZNF51
Gene Symbol
BCL6
Additional Bcl-6 Products
Product Documents for Bcl-6 Antibody (BCL6/1982) [CoraFluor™ 1]
Product Specific Notices for Bcl-6 Antibody (BCL6/1982) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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