Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4A9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Bcl-xL Antibodies »

Product Specifications

Immunogen

Recombinant fragment expressed in E.coli corresponding to amino acids 1-212 of human Bcl-XL

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

25.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bcl-xL Antibody (OTI4A9) - Azide and BSA Free

Western Blot: Bcl-xL Antibody (OTI4A9)Azide and BSA Free [NBP2-70247]

Western Blot: Bcl-xL Antibody (OTI4A9)Azide and BSA Free [NBP2-70247]

Western Blot: Bcl-xL Antibody (OTI4A9) - Azide and BSA Free [NBP2-70247] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2L1 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2L1 (1:500)
Immunocytochemistry/ Immunofluorescence: Bcl-xL Antibody (OTI4A9) - Azide and BSA Free [NBP2-70247]

Immunocytochemistry/ Immunofluorescence: Bcl-xL Antibody (OTI4A9) - Azide and BSA Free [NBP2-70247]

Immunocytochemistry/Immunofluorescence: Bcl-xL Antibody (OTI4A9) - Azide and BSA Free [NBP2-70247] - Staining of HT29 cells using anti-BCL2L1 mouse monoclonal antibody
Immunohistochemistry-Paraffin: Bcl-xL Antibody (OTI4A9) - Azide and BSA Free [NBP2-70247]

Immunohistochemistry-Paraffin: Bcl-xL Antibody (OTI4A9) - Azide and BSA Free [NBP2-70247]

Immunohistochemistry-Paraffin: Bcl-xL Antibody (OTI4A9) - Azide and BSA Free [NBP2-70247] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BCL-XL mouse monoclonal antibody (1:50).
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Applications for Bcl-xL Antibody (OTI4A9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:200 - 1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl-xL

Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2 (2). It has been found that eterodimerization with Bax does not seem to be required for anti-apoptotic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis.

Long Name

B Cell Lymphoma/Leukemia x Long Form

Alternate Names

BclxL

Gene Symbol

BCL2L1

Additional Bcl-xL Products

Product Documents for Bcl-xL Antibody (OTI4A9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Bcl-xL Antibody (OTI4A9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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