beta-Catenin Antibody (6F9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53360

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Bovine, Canine, Chicken

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6F9

Concentration

0.2 mg/ml

View all beta-Catenin Antibodies »

Product Specifications

Immunogen

Recombinant chicken beta-Catenin (Uniprot: P35222)

Localization

Cell surface and Cytoplasmic

Specificity

This monoclonal antibody recognizes a protein of 92kDa, which is identified as beta-catenin. It shows no cross-reaction with gamma-catenin (also known as plakoglobin). The catenins, alpha, beta and gamma bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. Alpha-catenin was initially described as an E-cadherin-associated protein, and has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin. Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. Beta-catenin has also been found in complexes with the tumor suppressor protein APC.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54543)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for beta-Catenin Antibody (6F9)

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6F9) [NBP2-53360]

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6F9) [NBP2-53360]

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (6F9) [NBP2-53360] - Confocal immunofluorescence image of HeLa cells using beta-Catenin Antibody (6F9). Green (CF488) and Red Dot is used to label the nuclei.
Immunohistochemistry-Paraffin: beta-Catenin Antibody (6F9) [NBP2-53360]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (6F9) [NBP2-53360]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (6F9) [NBP2-53360] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin (p120) Monoclonal Antibody (6F9).

Applications for beta-Catenin Antibody (6F9)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

UniProt

P35222

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (6F9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-Catenin Antibody (6F9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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