beta-Catenin Antibody (OTI12H7) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70509CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP2-03615

Alexa Fluor 350

NBP2-70509AF350

Alexa Fluor 405

NBP2-70509AF405

Alexa Fluor 488

NBP2-70509AF488

Alexa Fluor 532

NBP2-70509AF532

Alexa Fluor 594

NBP2-70509AF594

Alexa Fluor 647

NBP2-70509AF647

Alexa Fluor 700

NBP2-70509AF700

Alexa Fluor 750

NBP2-70509AF750

Allophycocyanin

NBP2-70509APC

Biotin

NBP2-70509B

DyLight 350

NBP2-70509UV

DyLight 405

NBP2-70509V

DyLight 488

NBP2-70509G

DyLight 550

NBP2-70509R

DyLight 594

NBP2-70509DL594

DyLight 650

NBP2-70509C

DyLight 680

NBP2-70509FR

DyLight 755

NBP2-70509IR

FITC

NBP2-70509F

HRP

NBP2-70509H

Janelia Fluor 525

NBP2-70509JF525

Janelia Fluor 549

NBP2-70509JF549

Janelia Fluor 585

NBP2-70509JF585

Janelia Fluor 635

NBP2-70509JF635

Janelia Fluor 646

NBP2-70509JF646

Janelia Fluor 669

NBP2-70509JF669

mFluor Violet 450 SE

NBP2-70509MFV450

mFluor Violet 500 SE

NBP2-70509MFV500

mFluor Violet 610 SE

NBP2-70509MFV610

PE

NBP2-70509PE

PerCP

NBP2-70509PCP

Forumulation

Catalog #

Azide and BSA Free

NBP2-70509

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI12H7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all beta-Catenin Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CTNNB1 (NP_001895) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for beta-Catenin Antibody (OTI12H7) [CoraFluor™ 1]

Application

Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (OTI12H7) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for beta-Catenin Antibody (OTI12H7) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

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