beta-Catenin Antibody (OTI12H7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70509

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI12H7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all beta-Catenin Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CTNNB1 (NP_001895) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

85.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-Catenin Antibody (OTI12H7) - Azide and BSA Free

Western Blot: beta-Catenin Antibody (OTI12H7)Azide and BSA Free [NBP2-70509]

Western Blot: beta-Catenin Antibody (OTI12H7)Azide and BSA Free [NBP2-70509]

Western Blot: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta Catenin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-beta Cate
Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509]

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509]

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY beta Catenin.
Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509]

Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509]

Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.
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Applications for beta-Catenin Antibody (OTI12H7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:1000-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (OTI12H7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-Catenin Antibody (OTI12H7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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