beta-Catenin Antibody (SA30-04)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67307

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Multiplex Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SA30-04

Concentration

1 mg/ml

View all beta-Catenin Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human beta-Catenin aa 30-70. (SwissProt: P35222 Human; SwissProt: Q02248 Mouse; SwissProt: Q9WU82 Rat)

Localization

Cytoplasm, Nucleus, Cell membrane, Cell junction

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta-Catenin Antibody (SA30-04)

Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on HT-29 cell lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Immunocytochemistry: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Immunocytochemistry: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Usage of Beta Catenin antibody in ICC of HT-29 cells
Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Beta-Catenin with Rabbit anti-Beta-Catenin antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
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Applications for beta-Catenin Antibody (SA30-04)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:20-1:50

Multiplex Immunoassay

Multiplex IHC: 1:2000

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (SA30-04)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-Catenin Antibody (SA30-04)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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