beta-Catenin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89989
Key Product Details
Validated by
Orthogonal Validation, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Fish
Cited:
Fish
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAH
Reactivity Notes
Fish reactivity reported in scientific literature (PMID: 25502575).
Marker
Epithelial Cell Marker, Adherens Junction Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for beta-Catenin Antibody
Western Blot: beta-Catenin Antibody [NBP1-89989]
Western Blot: beta-Catenin Antibody [NBP1-89989] - Omegaven inhibits epithelial to mesenchymal transition induced by TGFbeta1.Human liver epithelial cell line THLE-3 was incubated in presence or absence of lipid emulsions Omegaven 10%, Lipofundin MCT/LCT 20%, ClinOleic 20% or SMOFlipid 20% at different dilutions, for 30 min followed by TGFbeta1 5 ng/mL stimulation for additional 72 hours 25 min. Phosphorylation of Samd3, ERK1/2 and Akt and nuclear expression of beta-Catenin. Representative western blot are showed and quantified in graphic bars. Results are expressed as means +/- SEM of six independent experiments. *p<0.05 related to the control group. #p<0.05 related to the stimulus. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0115404), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-89989]
Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-89989] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.Applications for beta-Catenin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: beta-Catenin
Additional beta-Catenin Products
Product Documents for beta-Catenin Antibody
Product Specific Notices for beta-Catenin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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