beta-Catenin [p Ser675] Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05452

Novus Biologicals, part of Bio-Techne
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NBP3-05452
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all beta-Catenin Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S675 of human beta-Catenin (NP_001091679.1). RLSVE

Modification

p Ser675

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta-Catenin [p Ser675] Antibody - Azide and BSA Free

Western Blot: beta-Catenin [p Ser675] AntibodyAzide and BSA Free [NBP3-05452]

Western Blot: beta-Catenin [p Ser675] AntibodyAzide and BSA Free [NBP3-05452]

Western Blot: beta-Catenin [p Ser675] Antibody [NBP3-05452] - Western blot analysis of extracts of C6 cells, using beta-Catenin antibody (NBP3-05452) at 1:1000 dilution. C6 cell lysates were treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.
beta-Catenin [p Ser675] Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: beta-Catenin [p Ser675] Antibody - Azide and BSA Free [beta-Catenin] -

Immunocytochemistry/ Immunofluorescence: beta-Catenin [p Ser675] Antibody - Azide and BSA Free [beta-Catenin] - Immunofluorescence analysis of Rat colon(treated with lambdaPP) and Rat colon tissue using beta-Catenin Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.
beta-Catenin [p Ser675] Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: beta-Catenin [p Ser675] Antibody - Azide and BSA Free [beta-Catenin] -

Immunocytochemistry/ Immunofluorescence: beta-Catenin [p Ser675] Antibody - Azide and BSA Free [beta-Catenin] - Immunofluorescence analysis of Mouse colon(treated with lambdaPP) and Mouse colon(untreated) tissue using beta-Catenin Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

Applications for beta-Catenin [p Ser675] Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin [p Ser675] Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for beta-Catenin [p Ser675] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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