BTLA/CD272 Antibody (OTI2E4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45549
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0002.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
![Western Blot: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Western-Blot-NBP2-45549-img0015.jpg "Western Blot: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI2E4
Concentration
1 mg/ml
Product Summary for BTLA/CD272 Antibody (OTI2E4)
Immunogen
Full length human recombinant protein of human BTLA(NP_861445) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
32.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BTLA/CD272 Antibody (OTI2E4)
Western Blot: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Western Blot: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0014.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Flow-Cytometry-NBP2-45549-img0001.jpg "Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Flow Cytometry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of live Sup-B15 cells, using BTLA antibody , compared to IgG1 isotype control (Black).![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0003.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0004.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0005.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0006.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0007.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0008.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0009.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0010.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0011.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0012.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0013.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Applications for BTLA/CD272 Antibody (OTI2E4)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using NBP2-45549 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BTLA
Long Name
B- And T-Lymphocyte Attenuator
Alternate Names
CD272
Gene Symbol
BTLA
Additional BTLA Products
Product Documents for BTLA/CD272 Antibody (OTI2E4)
Product Specific Notices for BTLA/CD272 Antibody (OTI2E4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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