BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72427
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0003.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
![Western Blot: BTLA/CD272 Antibody (OTI2E4)Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Western-Blot-NBP2-72427-img0001.jpg "Western Blot: BTLA/CD272 Antibody (OTI2E4)Azide and BSA Free [NBP2-72427]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI2E4
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human BTLA(NP_861445) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
32.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free
Western Blot: BTLA/CD272 Antibody (OTI2E4)Azide and BSA Free [NBP2-72427]
Western Blot: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0014.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72427-img0002.jpg "Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]
Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of live Sup-B15 cells, using BTLA antibody , compared to IgG1 isotype control (Black).![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0004.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0005.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0006.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0007.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0008.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0009.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0010.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0011.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0012.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0013.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]](https://resources.bio-techne.com/images/products/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0015.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]")
Applications for BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Western Blot
1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BTLA
Long Name
B- And T-Lymphocyte Attenuator
Alternate Names
CD272
Gene Symbol
BTLA
Additional BTLA Products
Product Documents for BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free
Product Specific Notices for BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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