BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72427

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2E4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all BTLA Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BTLA(NP_861445) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

32.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free

Western Blot: BTLA/CD272 Antibody (OTI2E4)Azide and BSA Free [NBP2-72427]

Western Blot: BTLA/CD272 Antibody (OTI2E4)Azide and BSA Free [NBP2-72427]

Western Blot: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]

Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]

Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]

Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427]

Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of live Sup-B15 cells, using BTLA antibody , compared to IgG1 isotype control (Black).
View 12 more images

Applications for BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BTLA

BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 (MIM 600244) and CTLA4 (MIM 123890), BTLA interacts with a B7 homolog, B7H4.[supplied by OMIM]

Long Name

B- And T-Lymphocyte Attenuator

Alternate Names

CD272

Gene Symbol

BTLA

Additional BTLA Products

Product Documents for BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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