Caldesmon/CALD1 Antibody (CALD1/820 + h-CALD)

Validated:

Unconjugated

Monoclonal Mouse IgG1 Kappa/IgG1 Kappa Clone # CALD1/820 + h-CALD

0.2 mg/ml

Recombinant full-length human Caldesmon/CALD1 protein (Caldesmon/CALD1/820); Crude human uterus extract (h-CALD) (Uniprot: Q05682)

Cytoplasmic

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120'150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70'80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This monoclonal antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Smooth Muscle Marker

Monoclonal

Mouse

IgG1 Kappa/IgG1 Kappa

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47819)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Protein A or G purified

10 mM PBS with 0.05% BSA

0.05% Sodium Azide

0.2 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C.