Calponin 1 Antibody (CNN1/4227R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07589

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # CNN1/4227R

Concentration

0.2 mg/ml

View all Calponin 1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues on the C-terminus of human Calponin 1 was used as an immunogen. (Uniprot: P51911)

Localization

Cytoplasmic

Specificity

Calponin a 33 kDa polypeptide, is a cytoskeleton-associated actin-binding protein that also interacts tropomyosin and calmodulin. Calponin antibody has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin antibody may also have applications in malignant myoepithelium and pleomorphic adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions. The antibody has been found in tissue extracts from smooth muscle, but not in fibroblast extracts.

Marker

Smooth Muscle Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08462)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Calponin 1 Antibody (CNN1/4227R)

Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589]

Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589]

Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589] - Formalin-fixed, paraffin-embedded human leiomyosarcoma stained with Calponin 1 Rabbit Recombinant Monoclonal Antibody (CNN1/4227R).
Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589]

Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589]

Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589] - Formalin-fixed, paraffin-embedded human leiomyosarcoma stained with Calponin 1 Rabbit Recombinant Monoclonal Antibody (CNN1/4227R).

Applications for Calponin 1 Antibody (CNN1/4227R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Calponin 1

Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).

Alternate Names

Calponin H1, CNN1, Sm-Calp, SMCC

Gene Symbol

CNN1

UniProt

P51911 (Human)

Additional Calponin 1 Products

Product Documents for Calponin 1 Antibody (CNN1/4227R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calponin 1 Antibody (CNN1/4227R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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